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13 thoughts on “C11 – Sacconi Nunez”
What further experiments could be done aside from those presented?
Hey, this was a wonderful presentation. If you were to do a western or northern blot, is there any specific protein or mRNA that you would be looking for?
If Socax is found to be a DNA repair gene in further experiments, then what would be the implications of this finding? How could this finding be used in further research?
Once it is established that the DNA does play a role in DNA damage repair, we could test how it is involved. We could see if it is involved in repairing double stranded breaks, or DNA polymerase mechanisms, DNA and chromatin dynamics, among others. Many experiments would have to be done to be able to test this, of course, but it cold get us closer to understanding DNA damage repair mechanisms.
what experiments could be done if you continued your research ?
What results would you need to see to be able to determine if the Socax gene plays a role in DNA damage?
If Socax does play a role in DNA damage repair mechanisms, in the gel we would see a brighter band for the cells treated with HU, as that would indicate that the expression of the gene increased in response to DNA damage. Remember, brighter bands means more expression.
If socax gene was involved in DNA damage repair mechanisms, we would have seen a brighter band for cells treated with HU, compared to untreated cells. A brighter band would indicate that expression of the gene increased in response to DNA damage. If the gene’s expression increases with DNA damage, we can deduce that it is involved in DNA damage.
Would you expect to receive concluding results if you were to repeat your second amplification considering the primers were viable?
Using primers that were already validated, and using good reagents should get us more definite results. R
When replicating this experiment, we would hopefully see bands for socax gene for untreated and treated cells. Depending on the bands that we see (the relative intensity of these bands) we would be able to determine if socax plays a role or not.
I was wondering what your controls were that were used in your first experiment?
The Positive controls for the first experiment were ftt18 gene cDNA and gDNA, as we already knew what length they were supposed to be, therefore we knew where the bands were supposed to be. Seeing the bands where the were supposed to, showed us that the PCR and Gel electrophoresis set ups were correct. The negative control was water with ftt18 and water with socax because with water, we are not supposed to see any bands. If we saw bands, that was an indication of contamination in the gel.
my lab was very different than this one so I am just wondering how exactly would you design primers that would anneal to the DNA sequence?