Or when running the experiment did you consider letting the PCR run for longer to provide higher concentrations of the target to see if there was a more visible difference in the expression?
When you run a PCR for a certain amount of time, it reaches a threshold amount to where it becomes saturated enough and it wouldn’t really matter if you ran it for longer.
These genes are of interest to us because they can help us understand how we could repair DNA in our own bodies. This could help us treat cancers and other genetic diseases.
For future directions, how would more reverse transcriptase PCR and gel electrophoresis specifically show that Corey is involved in the DNA damage repair pathway?
We have only one experiment here that confirms our results, and it would be beneficial to have more data to support our claim. Along with this, we could also do quantitative RT-PCR which could give us numbers to support the slight increase in expression that we see here. The gel electrophoresis helps us visualize our results for the RT-PCR, but is not needed for the qRT-PCR, which gives you numerical data.
Or when running the experiment did you consider letting the PCR run for longer to provide higher concentrations of the target to see if there was a more visible difference in the expression?
When you run a PCR for a certain amount of time, it reaches a threshold amount to where it becomes saturated enough and it wouldn’t really matter if you ran it for longer.
For about how long did you let the PCR run for? And do you think a longer length would make a difference?
We ran the PCR for 35 cycles. This was enough for it to become saturated enough to have it not really matter if you ran it any longer.
Why are genes involved in DNA repair of interest to us? How could it be helpful in the future?
These genes are of interest to us because they can help us understand how we could repair DNA in our own bodies. This could help us treat cancers and other genetic diseases.
For future directions, how would more reverse transcriptase PCR and gel electrophoresis specifically show that Corey is involved in the DNA damage repair pathway?
We have only one experiment here that confirms our results, and it would be beneficial to have more data to support our claim. Along with this, we could also do quantitative RT-PCR which could give us numbers to support the slight increase in expression that we see here. The gel electrophoresis helps us visualize our results for the RT-PCR, but is not needed for the qRT-PCR, which gives you numerical data.
For your future directions, how would more DNA transcriptase PCR and gel electrophoresis show that Corey is involved in the DNA damage repair pathway?