12 thoughts on “C15 – Callaway

  1. How did you select the Hymelc gene as the gene that you tested? Was it because it has high expression during early conjugation, or were there other factors that made it a promising choice?

    1. We were assigned specific genes and then researched their expression patterns; I did not know about Hymelc’s expression patterns during conjugation until after it was assigned, and most of the other information about Hymelc (domains, homologs) was actually not very promising in the scope of this research.

    1. Brighter bands show higher expression, so DNA that had been damaged with Hydroxyurea would show higher expression patterns (brighter bands) if my gene was involved in DNA damage repair, and thus was more active in the presence of damage.

  2. You said that the annealing happened right where you expected it and where it was supposed to, but where is that exactly in more detail? Great job!

    1. For my primers, I expected them to anneal at 410bp to both cDNA and gDNA, as this is where I designed them to anneal over digital software. The other primers (my control) had annealing sites specific to that gene’s primers. Correct primer annealing ensures that my primers did not have sequence problems or form dimers.

    1. The function of the gene is still unknown, as my research showed that it is not involved in DDR. There is very little additional information on the Hymelc gene, so research for individual cell functions would most likely help to determine the function of this gene. All that is currently known is that Hymelc has high expression patterns during early and late conjugation that is most likely not important in DNA damage repair.

  3. Do you have any hypotheses about other purposes that the Hymelc gene may serve if not DNA damage repair?

    1. Since there is not a lot known about Hymelc other than its conjugation expression patterns, not really. There is very little information known on this gene which made hypothesizing the results of my experiments pretty much a random shot.

  4. Do you have any other hypotheses about what the Hymelc gene’s purpose is if not in the DNA damage repair pathway?

  5. With your knowledge of the Hymelc gene how will this benefit your research moving forward? How will you use this information?

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