6 thoughts on “C19 – Allen

  1. Hi Madison! Great presentation! As a Drug Discovery student, I am curious as to how you read your results in Figure 4. I understand Figure 4 suggests a successful binding of gene specific primers but how is this read from the data? Just curious!!

    1. In order to read the results of figure 4, you would look at the different expression of the band and make sure that they would bind at the appropriate base pairs without any contamination in your negative control. In our figure, we have contamination in our positive control showing that the cDNA mixed in with the other samples. We know this because cDNA binds at 302 bp and it shows powerful bands in all of those lanes around 302bp. Since this didn’t happen for our gene specific data and only our housekeeping gene, we could still use these primers!

  2. Beautiful poster and presentation! I am curious to know what kind of environmental conditions cause damage to the DNA and did any of your classmates find the opposite of your findings where the damage to the DNA actually increased the expression of a gene?

    1. Some of my classmates had similar results to mine meaning that our gene is not involved in the DNA damage cycle, but perhaps is involved in another type of repair mechanism in conjugation. Some of my peers did have expression so this would mean that it is involved in DNA damage repair. Some ways that DNA can be damaged are through intracellular metabolism, replication problems, exposure to genotoxic agents, etc.

  3. You mentioned in your future directions that you’d seek to elucidate the structure of Upec via Cryo-EM. What’s Cryo-EM?

    1. Cryo-EM is Cryogenic Electron Microscopy in which samples are cooled to cryogenic temperatures and embedded in an environment of vitreous water. It helps illuminate large complex components of cell biology and microbiology.

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