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8 thoughts on “C19 – Antonucci”
Hi! I was wondering, is your gene of interest known to serve other functions in any organisms?
Hi there! As far as I could tell when researching my gene of interest, AMP binding proteins typically exist in bacterium, but there are other similar organisms, such as humans that have a very similar proteins present (cyclic AMP). But, there was no indication of my gene of interest serving any other function in organisms as it hasn’t been studied very much.
what different kind of experiments can be done to further the research?
Some experiments I may suggest to further the research for this specific gene may be inducing DNA damage during a different stage of conjugation or preconjugation to see if my gene of interest is involved in processes before DDR or after DDR is induced. Or, to test whether or not the gene is involved in the Kreb’s cycle or glycolysis by testing cell growth via the concentration of ATP to AMP in the cell.
How did you reliably damage the DNA for your experiment?
DNA was damaged by taking tetrahymena cells and centrifuging them in a solution of hydroxyurea and letting the cells sit for a given amount of time (I believe a few hours to a day) to make sure DSBs were induced correctly.
Hi! Really good presentation! I was wondering why you chose the gene you did for this research.
Thank you very much! I didn’t really pick this gene as it was more assigned to me but it was a viable candidate because in figure 2 of my poster the gene specific graph shows two areas of conjugation that have increased levels of mRNA which indicated that there was a possibility of increased expression during DDR.