It was not too difficult since there are good tools available to simplify the process. We got the sequence data from ciliates.org and used Primer3Plus to find good matches.
So there isn’t much at all known about this gene, but based on the little information available I would think it plays a role in the conjugation cycle but it is not involved in DNA repair. I tried to put the sequence through protein folding software but I did not get any good matches.
So there isn’t much at all known about this gene, but based on the little info available I would say it plays some role in the conjugation process, just not DNA repair related.
What other types of genes besides DNA damage repair genes are expressed during the conjugation cycle in a similar way to Hymelc, and do you think it’s possible that Hymelc’s function could be related to any of those?
There are a number of different types of genes that are expressed during conjugation, many are related to the processes that take place like the pairing of cells, meiosis, and nuclear differentiation that I think Hymelc could play a role in.
How did you know how many base pairs to expect in your products?? Is it just based off of prior experimentation. I may have missed it in your presentation, but great job!
Fortunately the T. thermophila genome has been previously annotated so I could look up the size and sequence of the gene and how many introns it had (0).
Was it super difficult to design primers?
It was not too difficult since there are good tools available to simplify the process. We got the sequence data from ciliates.org and used Primer3Plus to find good matches.
If you could predict the function of the gene based off of what you do know, what would you say?
So there isn’t much at all known about this gene, but based on the little information available I would think it plays a role in the conjugation cycle but it is not involved in DNA repair. I tried to put the sequence through protein folding software but I did not get any good matches.
So there isn’t much at all known about this gene, but based on the little info available I would say it plays some role in the conjugation process, just not DNA repair related.
What other types of genes besides DNA damage repair genes are expressed during the conjugation cycle in a similar way to Hymelc, and do you think it’s possible that Hymelc’s function could be related to any of those?
There are a number of different types of genes that are expressed during conjugation, many are related to the processes that take place like the pairing of cells, meiosis, and nuclear differentiation that I think Hymelc could play a role in.
What areas of this experiment would you like to change or improve on if you were to redo it?
I would’ve liked to measure gene expression again and quantify it via qPCR to get a more robust measurement.
How did you know how many base pairs to expect in your products?? Is it just based off of prior experimentation. I may have missed it in your presentation, but great job!
Fortunately the T. thermophila genome has been previously annotated so I could look up the size and sequence of the gene and how many introns it had (0).