Difficult to say, the primer3plus program predicated they would anneal but it’s just a prediction. It may have been an issue with the melting temp (ideal was 55 centigrade but primers were 52.3), or the sequence was too heavy in G/C base pairs
I think I’m most interested in the response aspect of reparation – what signals the cell to get things moving and realize there is a break/issue? I’d like to pursue that more
Why did the specific gene primers fail to anneal?
Difficult to say, the primer3plus program predicated they would anneal but it’s just a prediction. It may have been an issue with the melting temp (ideal was 55 centigrade but primers were 52.3), or the sequence was too heavy in G/C base pairs
Great presentation! Whats another aspect of gene reparation that you would like to study?
I think I’m most interested in the response aspect of reparation – what signals the cell to get things moving and realize there is a break/issue? I’d like to pursue that more