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8 thoughts on “C26 – Orona”
Was there any previous research on your gene?
No previous research had been done on Hypr2c outside of the expression profile I provided in my poster in addition to the ciliates.org entry with the genes TTHERM # in the references section of the poster. As a result, most of the future directions aimed to investigate some of the more fundamental attributes of Hypr2c as even the presence of homolog sequences were scarce.
Hi, great job on this project!! What made you decide to use Rad51 and Ftt18 as the control genes?
While many genes are upregulated (and even more neutral) in the presence of DNA damage, Rad51 and Ftt18 are present in a variety of species and most of the information pertaining to their role in the DNA damage response and repair pathway comes from many research papers where one, the other, or both genes are the subject.
Did this gene prove to have significant expression in other DNA pathways within the body? Could you devise further experimentation to prove that?
Any future directions aside from the ones I included would have to address a potential pathway Hypr2c could play a role in. There is not much information on this gene to begin with, and in trying to find evidence for a specific pathway, I would likely utilize the BAC protein assay following analysis of the t, thermophila genome in order arrow down a potential pathway for inquiry. My conclusion would encompass data from bioinformatic analysis to determine the gene surrounding Hypr2c in combination with the presence or absence of Hypr2c protein attributes that are typical (or atypical) of that chosen pathway.
Good Job on the presentation! Do you know more information on how the primers were specifically designed?
How are you sure that the Hypr2c gene is present in any pathways if it isn’t in the DNA damage/repair pathway? or is it just hypothetical?