Our primer that is supposed to increase expression under condition of DNA damage did not. We have a couple ideas such as contamination of DNA damaging agents in the untreated sample but we are not sure why it is the way it is.
We used a program that took in the DNA sequence of our gene and produces potential primer pairs. We then chose whichever primers were best using various criteria
What were the unexpected results from your experiment that made it so you couldn’t get results? Solely DNA damage or something else?
Our primer that is supposed to increase expression under condition of DNA damage did not. We have a couple ideas such as contamination of DNA damaging agents in the untreated sample but we are not sure why it is the way it is.
How did you make primers that were specific Hyplrr?
We used a program that took in the DNA sequence of our gene and produces potential primer pairs. We then chose whichever primers were best using various criteria
How do you obtain/administer the Rad51?
The Rad51 primers were provided to us.