Hey Lillie!
It is likely that my gene will be ruled out for further research concerning the DNA damage repair pathway. However, it is possible that further research can be conducted in order to determine the genes specific function in the cell.
RPP0 acted as a control to make sure we were setting up the PCR reactions correctly, and that equal amounts of mRNA were added in RT-PCR. Rad51 primers were used because Rad51 is a gene known to be involved in DNA damage repair. This also acted as a control, and gave us an idea of an expected result if mRNA expression increased during DNA damage repair. The MMH primers were used to isolate the gene of interest.
Great presentation! How can your research be used in future research projects in this field? based on the results you got what do you assume is the function of your gene?
It is not likely that my gene is a good candidate for further research concerning the DNA damage repair pathway. That being said, its hard to predict any other function in the cell based solely on my research. Previous studies have shown potential ATP binding and ribonucleoside-diphosphate reductase activity. It may also be involved with oxidation-reduction processes or DNA replication!
How do you think the gene you studied in this project can be used to further other research in this field?
Hey Lillie!
It is likely that my gene will be ruled out for further research concerning the DNA damage repair pathway. However, it is possible that further research can be conducted in order to determine the genes specific function in the cell.
I may have missed it, but why was the faint band not supposed to be present/ why was it unexpected when you saw a band there?
I apologize I got my questions mixed up, my question for your poster was what lead you to choose the primers used?
RPP0 acted as a control to make sure we were setting up the PCR reactions correctly, and that equal amounts of mRNA were added in RT-PCR. Rad51 primers were used because Rad51 is a gene known to be involved in DNA damage repair. This also acted as a control, and gave us an idea of an expected result if mRNA expression increased during DNA damage repair. The MMH primers were used to isolate the gene of interest.
also no worries about the mixup!
Hey Elias!
I assume you’re referring to the faint bands at the bottom of the gels, which are generally primers!
Great presentation! How can your research be used in future research projects in this field? based on the results you got what do you assume is the function of your gene?
It is not likely that my gene is a good candidate for further research concerning the DNA damage repair pathway. That being said, its hard to predict any other function in the cell based solely on my research. Previous studies have shown potential ATP binding and ribonucleoside-diphosphate reductase activity. It may also be involved with oxidation-reduction processes or DNA replication!