10 thoughts on “C28 – MacGuire

  1. Did you find any genes that are sequence homologs to Rfc1 and if so what can they tell you about its potential function?

    1. I Did! Rfc-1 has a human homolog by the same name which assists in the loading of DNA polymerase onto the DNA strand.

  2. How would you specifically analyze phenotypic changes (in regards to your future directions)?

    1. This could just be as simple as making the gene inaffective and seeing if that alters the DNA damage repair pathway.

  3. This was a really good presentation! If you were able to investigate potential phenotypic changes as a result of Rfc1 in the future, would you do so in Tetrahymena thermophila as well, or would you do it in a different organism?

    1. I would probably do it in T. thermophila just because it would be great model organism for this expeirment!

  4. How likely is it that DNA damage causes up/down regulation in genes that are NOT involved with repair, such that you can conclude that any upregulated gene in the presence of DNA damage is likely involved with repair?

    1. That’s a really good question! I don’t know how likely it is but keep in mind that genes picked for this expirement are ones that have those two increases in expression during conjugation which points in the direction of involvement in damage repair. Ultimately there are may expriments that need to take place in order to determine this and this is just one of those that says it is very possible that Rfc1 maybe involved in the DNA damage repair pathway.

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