10 thoughts on “C29 – Milovska

  1. Since you guys had results showing that Tetco is a DNA repairing gene, for your further directions what you recommend to test Tetco for further testing of DNA replications?

    1. Great question! I would actually want to replicate our study first, since we had technical issues 🙂 and drew our conclusions based on the consensus gel. Assuming we were able to replicate the consensus gel results though, I would want to know what Tetco61 is binding to and the cellular pathway it’s part of using cell-free assays.

    2. Wait. Now I’m not so sure I answered your question. If you’re asking how we can be sue Tetco61 is a DNA repairing gene, well, then we could damage cellular DNA with something other than hydroxyurea and measure Tetco61 expression levels again.

  2. What do you propose as a method for future research into the role of Tetco61? Would you suggest replicating your experiments, or is there anything you would change?

    1. I would definitely start with replicating the experiment, since we ended up using the consensus gel for our conclusions rather than our own results due to experimental errors. Assuming replication confirms the results seen in the consensus gel, I would want to find out what Tetco61 binds to and its mechanism of action. That would perhaps best be accomplished via a cell-free assay.

  3. You mentioned you chose Tetco61 specifically since it could be involved in damage repair, do you know of any other genes that could be involved in this process as well?

    1. We chose Tetco61 because it is upregulated in T. thermophila conjugation, when a lot of DNA damage is induced and subsequently repaired. A gene known to be involved in DNA damage repair is Rad51, which we used as a positive control in our study.

  4. If Tetco61 was determined to be involved in DSB repair, what implications would this have for medical treatment for related diseases?

  5. If tetco61 was determined to be involved in DSB repair, what implication would this have for treatment for related diseases?

    1. If it were human diseases we were talking about, it would depend on whether homologous genes of Tetco61 exist in the human genome. We actually didn’t find any homology in any other species in BLAST. So the utility of this gene but be primarily as a research tool.

Leave a Reply