There was likely no contamination in the cDNA for our specific primer. The experiment and figure 8 was done mainly to see how well the primer we design anneals to our specific gene of interest. When compared to the already validated primer, the band was brighter which indicates that The primer we designed does not Anneal as well to our gene of interest.
There was likely no contamination in the cDNA for our specific primer. The experiment and figure 8 was done mainly to see how well the primer we design anneals to our specific gene of interest. When compared to the already validated primer, the band was brighter which indicates that The primer we designed does not anneal as well to our gene of interest. However, contamination may have occurred but replicate experiments would have to be performed to test this possibility.
There was likely no contamination in the cDNA for our specific primer. The experiment and figure 8 was done mainly to see how well the primer we design anneals to our specific gene of interest. When compared to the already validated primer, the band was brighter which indicates that the primer we designed does not Anneal as well to our gene of interest. The only issue that could have impacted are results may be that not enough DNA was loaded into the wells.
The design primers were not considered valid because our band was significantly dimmer then the already validated primer band. From that we decided to utilize the validated primer in further experiments to ensure that we get the best results.
We hypothesized that Hyplrr plays a significant role in the DNA damage pathway because it displays a similar expression pattern as a known gene (Rad51) that plays a role in the DNA damage pathway.
Thank you! I believe more experiments should be done in order to have a stronger conclusion that Hyplrr is in fact involved in the DNA damage pathway and that there wasn’t any contamination in our experiment. A possible experiment could involve the use of a reporter gene to see exactly when and where Hyplrr is expressed. A knock-out of the gene can also be utilized to see if the gene plays an essential role in the DNA damage repair pathway.
I’m not sure which figure you are referring to, however, if you are referring to figure 8 then it is most likely gDNA contamination which could have occurred from the gel moving or maybe the tips were not changed when loading the wells.
Do you know why the cDNA for your gene specific primer in figure 8 had low expression? Was there any contamination?
There was likely no contamination in the cDNA for our specific primer. The experiment and figure 8 was done mainly to see how well the primer we design anneals to our specific gene of interest. When compared to the already validated primer, the band was brighter which indicates that The primer we designed does not Anneal as well to our gene of interest.
There was likely no contamination in the cDNA for our specific primer. The experiment and figure 8 was done mainly to see how well the primer we design anneals to our specific gene of interest. When compared to the already validated primer, the band was brighter which indicates that The primer we designed does not anneal as well to our gene of interest. However, contamination may have occurred but replicate experiments would have to be performed to test this possibility.
Why were the designed primers not valid?
There was likely no contamination in the cDNA for our specific primer. The experiment and figure 8 was done mainly to see how well the primer we design anneals to our specific gene of interest. When compared to the already validated primer, the band was brighter which indicates that the primer we designed does not Anneal as well to our gene of interest. The only issue that could have impacted are results may be that not enough DNA was loaded into the wells.
The design primers were not considered valid because our band was significantly dimmer then the already validated primer band. From that we decided to utilize the validated primer in further experiments to ensure that we get the best results.
What was the thought process in deciding to go with that certain hypothesis?
We hypothesized that Hyplrr plays a significant role in the DNA damage pathway because it displays a similar expression pattern as a known gene (Rad51) that plays a role in the DNA damage pathway.
What made you decide on that certain hypothesis
ignore this
Hi! amazing presentation. What experiments, if any, would be performed to test the role hyplrr plays in the DNA damage repair pathway?
Thank you! I believe more experiments should be done in order to have a stronger conclusion that Hyplrr is in fact involved in the DNA damage pathway and that there wasn’t any contamination in our experiment. A possible experiment could involve the use of a reporter gene to see exactly when and where Hyplrr is expressed. A knock-out of the gene can also be utilized to see if the gene plays an essential role in the DNA damage repair pathway.
I really like your poster! What do you think led to your results being contaminated?
I’m not sure which figure you are referring to, however, if you are referring to figure 8 then it is most likely gDNA contamination which could have occurred from the gel moving or maybe the tips were not changed when loading the wells.