8 thoughts on “C30 – Rapetti

    1. So the spike that are present in figure one, demonstrates where the gene is being expressed to a greater extend in conjugation.

  1. Great job! Are there any other ways to determine the genes function besides measuring expression?

    1. So this may not be in the context of this lab, however other ways of determining gene function could be to inactivate the gene entirely and see what the response is.

  2. Great job! Are there any other ways to determine the genes function besides measuring expression?

    1. Yes so another person was also interested in this. One alternative would be to inactivate the gene entirely, using techniques such as CRISPR, and observe the changes that occur due to the inactivation of the gene.

  3. This was presented really well! What do you think you could have done differently to achieve different results the second time around?

    1. If I were to do this experiment again, for better presentation, not necessarily required, I would redo the primer validation experiment so that when present the validation results in could insight greater confidence in the viewer. Apart from that our experiments went fairly well and would simply redo the gene expression experiment to avoid the contamination, resulting in cleaner results.

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