8 thoughts on “C33 – Roeder

  1. Excellent Presentation Payton. What exactly Is your model organism I’ve never heard of it before and how did you guys obtained it ? is it found in Humans?

    1. T. thermophila is a single-celled eukaryote that is found in freshwater and is easy to grow in a lab. It was grown by our instructor (Dr. Moore), who then provided us with the cDNA and gDNA.

  2. Were there any determining factors in why you investigated the gene ribonucleoside diphosphate reductase subunit M2?

    1. We investigated it because its expression during conjugation follows the double peak pattern shown in figure 1 on my poster. This pattern is often found in genes known to be involved in DNA repair, such as Rad51, so we thought Rrm2 could also be involved in DNA repair.

    1. A knockout experiment involves deleting the gene from the genome so that its protein is no longer made. This means that whatever function the protein performs will no longer be happening. You can then look at the cells/organism and see what function(s) have been affected. Whatever function(s) are missing are what that protein does.

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