12 thoughts on “C35 – Trombly

    1. gDNA stands for genomic DNA, and cDNA stands for coding DNA. The difference is that cDNA has undergone splicing and pre-transcription modifications so that it can code for different genes.

  1. Would the results change significantly if you used another way of damaging the DNA other than hydroxyria? Or would this require a whole other experiment?

    1. It would likely require a different experiment. We used HU as it relates to our hypothesis of Rrm2’s ability to repair double-stranded breaks, which HU causes. It’s not impossible that the results would change significantly if we used a substance that caused single-stranded breaks.

    1. We chose HU because of its’ ability to specifically create double-stranded breaks as opposed to single-stranded or other DNA damage, as that related back to our hypothesis of repair in DSBs.

    1. It’s unclear as not a lot of experimentation on this gene has been done in the past, but some form of cell repair is likely.

  2. Great job! Your content was super interesting and you explained everything really well. One question I have is, what are the primers you’re talking about and what is PCR amplification?

    1. The primers i’m referring to are what we used to amplify our target gene for the experiment, and PCR amplification is the method of creating lots of copies of our specific DNA sequence that makes up the target gene.

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