9 thoughts on “C35 – Talder

  1. Awesome job on your presentation! I was wondering how exactly you would go about performing a gene knockout to visualize the DNA damage response pathway in the absence of the Hypzif?

    1. Great question. Becuase there are numerous factors that could affect the replication, to complete this, I would suggest sight directed mutagenesis to create a stop codon prematurely.

    2. I would perform a gene knockout by using site-directed mutagenesis to complete a premature stop. I am mostly speculating though.

  2. Good job! Once you perform protein localization and determine when and where Hypzif functions, how could you use this knowledge to affect the DNA damage response pathway to target cancer?

    1. By knowing the function and timing of Hypzif, we would be able to use this importation to allow to see for the exact gene that may need to be repaired. This is the future will be able to be specific to direct to specific genes.

  3. What are some functions you suspect the gene has based off of your background information and experiment results?

    1. Becuase there is a similar gene expression profile for those that Rad51 has, there is the possibility that Hypzif could be involved in the DNA damage repair pathway, but has low expression. By scaling up the concentration of the cDNA synthesis, this could be validated.

    1. The limitations where that the gDNA was degraded, and mostly timing. We needed to run more experiments to allow for an increase in reputable data. By completing another PCR we could have increased the concentration allowing to see the expression levels.

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