6 thoughts on “C39 – Berriman-Rozen

  1. What inclined you to use the gene UPEC?There is probably many genes that help with DNA repair, so why this one?

    1. Hi Ryan,

      The UPEC gene was identified as a candidate based on its expression profile, which matched known proteins that participate in the DDRP like Rad51. Because we are trying to find new genes, we have to basically screen model organisms to find them, rather than knowing which are involved beforehand.

  2. Since the compound you chose to further test on, since you knew it was upregulated, why did you chose the UPEC gene, did any previous studies done with your compound reveal what you may have concluded if it had succeeded? Also, you mentioned that you would not recommend further studies done with the compounds/genes you were working with, do you find that there could be a different conclusion with either the UPEC gene or Hydroxyurea? Great poster!

    1. Hi Paige,

      The UPEC gene was chosen because its expression profile looked similar to those known to participate in the DDR pathway. The UPEC gene product additionally has no known homologues or motifs which means it does not resemble known protein configurations, including those used in DDRP proteins. All in all, I don’t believe the UPEC gene presents value for further study. I will say, a more rigorous protocol involving hydroxyurea dose-response curves and qPCR could further cement or contradict this notion.

  3. Do you have any other potential gene candidates to study their impact in the gene damage repair pathway because your results were so surprising? If so, what are they and why?

    1. Hi Alex,

      Unfortunately, the scope of this project looked at a single candidate. I’m not quite sure how many candidates there are, however, DDR pathway proteins are highly conserved so there may be other eukaryotic DDRPs that could be studied as well, which obviously expands our playing field.

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