8 thoughts on “C4 – Batteau

  1. If the procedure for gel electrophoresis is done with perfection and no room for errors (hypothetical), what would change would be observed in the intensities of slot 2 and 5 of figure 3 with similar intensities?

    1. Consensus gels tell us that lanes 2 and 5 will have bands of equal intensity due to the fact that the primer was annealed to that specific gene sequence.

  2. If you were to repeat your experiments, what would you do to eliminate the possible experimental error?

    1. If experiments were repeated, we would make sure our PCR rig is set up correctly, and we would make sure to inject the same amount of material into each well.

  3. After you validate your results, what techniques will you use to figure out what the function of the SetK gene is? Then, what will you do with that information? Could knowing the function of the gene help find the cause of certain diseases or health problems?

    1. We could use CRISPR to get rid of the SetK gene and see how the cell functions without it. We could then possibly assign a function to the setk gene. Knowing the function of SetK could help us understand the mechanisms that could cure or prevent diseases.

  4. You mention comparing protein function and presence as a future direction. Do you think your gene can be modified to be more resistant to DNA damage? If so, what are some avenues you might pursue?

    1. I don’t think there is a way to make genes more resistant to DNA damage, however, if we understand the DNA damage repair mechanisms, we may be able to facilitate repair of damaged DNA which could help in future research for disease prevention

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