9 thoughts on “C40 – Campos

  1. Hey Abel!

    Great presentation! I was just curious about how during the process of DNA repair, is it more likely that homologous directed repair or nonhomologous end joining is used?

    1. Hi! I looked through the literature a little regarding that same question and mostly found mention of homologous directed repair, but no direct studied on the topic.

    1. We basically just looked at the sequence for our gene both with and without introns, and just came up with a series of sequences that would anneal on either side of our gene. We then used a website service called Pimer3+ which will send you primers if you send them the sequence.

  2. What was the specific process you used to create those custom primers? Overall great presentation!

  3. Your presentation was really interesting! I was wondering what tests you would perform to try to determine the function of the UPEC gene?

    1. Our group talked about potentially testing its expression under conditions that simulate different parts of the conjugation process, like for example testing UPEC’s expression in cells going through expression that are about to begin their DNA damage, to determine if UPEC might be involved in the process of damaging the genome, or something like that.

  4. Hi Abel! I loved your presentation. How would the UPEC gene behavior differ if it were involved in the DNA damage repair? What would your results look like?

    1. You would expect to see brighter bands from produced by the PCR using DNA from the cells with DNA damage, indicating that those cells were upregulating the UPEC gene in the presence of DNA damage.

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