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10 thoughts on “C41 – Boone”
In redoing the experiment, what would you change/add and why?
What do you think the significance of your experiment was and how would you use your research to further show the effects on DNA repair/ damage?
Unfortunately, the Socax does not have any homologs in humans, so this research might not be useful now in terms of cancer research, but the Tetrahymena have been less studied than other model organisms, so this research might have significance in future experiments on this organism.
Great job, Kirsten! If you repeat your experiment and see the same results as you’ve achieved thus far, what will that tell you about the cause of Socax gene expression? Will that clarify whether or not it’s a part of the DDR process, or activated for some other reason?
If the experiment was done again and the same results were found, then it would clarify that the Socax gene likely is involved in the DDR pathway, and that maybe my classmates doing the same experiment just had some procedural errors that lead to them not getting the same results.
Why did you use the socax in one part of the PCR and the Ftt18 in the other part?
Ftt18 was actually used in both, I just did not explain it that well. Ftt18 was the positive control in both parts to compare the results to and make sure everything was working properly.
what do you mean when you say “validate primers?”
Validating them just means that they work. Because we designed these own primers ourselves we did not know if they would be able to anneal during PCR. So if they work properly, they are valid.
How could you determine exactly what the role of Socax is in the DDR pathway?