Thank you! To design the primers we were given ideal criterion such as primer length and length of PCR product. These criterion were put into a website, primer3plus, which then generated five potential primers. I then chose one, and through PCR I was able to validate the primer.
DNA damage can be caused by an array of things in all organisms. In our experiment hydroxy urea was used to induce DNA damage, but things like UV damage can also create damage, which is often why skin cancers develop.
Often times errors are inevitable and data doesn’t necessarily turn out the way we want it to. To avoid PCR errors replication of the same experiment could be performed.
Great presentation! What do other future directions look like that our outside of your lab manual? Maybe some future directions that are currently doing more research?
In lab the final experiment detailed was the gene expression PCR and gel. Because my gel and PCR were inconclusive, several experiments could be performed. Running another PCR may give more clear results, but likely Hyproc14 is expressed at low levels unable be detected using this PCR. Going forward, a PCR experiment with greater detail could be useful in showing small changes in expression. Additionally, gene knockout therapy could be useful in showing Hyproc14’s function, if the gene is knocked out and the cell shows a change in phenotype this could indicate function.
I don’t believe that the RNA extraction itself was the error as it seems that the RNA extraction and conversion to cDNA worked well with Ftt18 and Rad51. I believe a PCR error occurred which may be due to smaller levels of cDNA present.
Great presentation! What do other future directions look like that our outside of your lab manual? Maybe some future directions that are currently doing more research?
Great presentation! How did you go about designing a primer for these studies?
Thank you! To design the primers we were given ideal criterion such as primer length and length of PCR product. These criterion were put into a website, primer3plus, which then generated five potential primers. I then chose one, and through PCR I was able to validate the primer.
Amazing job on your presentation! why did you use water for a negative control instead of something else?
Water is easy to access, making it an easy negative control to use. We also knew that there would be no reaction with water.
What are some factors which may cause DNA damage in T. thermophila?
DNA damage can be caused by an array of things in all organisms. In our experiment hydroxy urea was used to induce DNA damage, but things like UV damage can also create damage, which is often why skin cancers develop.
Great job! How could future research try to avoid pcr errors and make sure data is reliable?
Often times errors are inevitable and data doesn’t necessarily turn out the way we want it to. To avoid PCR errors replication of the same experiment could be performed.
Great presentation! What do other future directions look like that our outside of your lab manual? Maybe some future directions that are currently doing more research?
In lab the final experiment detailed was the gene expression PCR and gel. Because my gel and PCR were inconclusive, several experiments could be performed. Running another PCR may give more clear results, but likely Hyproc14 is expressed at low levels unable be detected using this PCR. Going forward, a PCR experiment with greater detail could be useful in showing small changes in expression. Additionally, gene knockout therapy could be useful in showing Hyproc14’s function, if the gene is knocked out and the cell shows a change in phenotype this could indicate function.
Do you have any insights about what could have gone incorrectly with the RNA extraction to lead to the trend that you observed?
I don’t believe that the RNA extraction itself was the error as it seems that the RNA extraction and conversion to cDNA worked well with Ftt18 and Rad51. I believe a PCR error occurred which may be due to smaller levels of cDNA present.
Great presentation! What do other future directions look like that our outside of your lab manual? Maybe some future directions that are currently doing more research?