9 thoughts on “C45 – Kiger

  1. Your presentation and poster look amazing! I was curious what types of diseases does DNA damage cause in people and/or animals?

    1. Thank you! And great question, the most important effect of DNA damage is mutations within the genome. This could result in either apoptosis or to diseases like cancer.

  2. Why do you recommend doing an RT-PCR for this experiment? What are you hoping to look for in terms of the RT-PCR if the normal PCR shows that Wcop40 is not increased during DNA damage?

    1. Great questions! An RT-PCR is done so that the RNA can be reverse transcribed back into cDNA. This allows us to measure the cDNA levels of each gene before and after DNA damage so that we can measure exactly how much the gene expression changed in each case in order to come to an answer to our central question. And to clarify, the results indicating the expression of Wcop40 did not change during DNA damage is the RT-PCR results, not a regular PCR results (the regular PCR was done for primer validation)!

    1. Thanks for the question! With there being no significant change in the gene Wcop40 expression after induced DNA damage, this indicates that Wcop40 is not significantly involved in the DNA damage repair process. If it was, the expression of the gene would increase significantly with DNA damage, which did not happen in this case.

    1. Good question! I would probably redo the primer validation PCR just for the sake of my conscious because there had been the faint band that showed up in the Atpv0c6 water lane, which should have had no bands in it since it was one of our controls. However, we had been able to explain the reason for that showing up since there had been a contamination in this same spot across all of the classes in our lab, which is why we had decided to use the results obtained still since there was a logical explanation for it.

  3. Great Presentation! Why do you think the Wcop40 did not increase after DNA damage was present as stated in your hypothesis?

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