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9 thoughts on “C46 – Kuna”
Great job! I’m a little bit confused about the role the reverse primer plays as mentioned in your methods section, could you explain that in more depth?
Hi! Thank you so much for positive feedback! Great question! So reverse primer is a primer that aniline to the end of a sequence that we want to multiplie by PCR and then look at it in gel electrophoresis. On the other hand side forward primer is a primer that aniline to the beginning of the sequence we want to multiply! hope it helped, if you have any more questions i wold be more than happy to answer them!
I see you said you were happy with your results because they were what you expected – could you respecify what was expected and how exactly that tied to your results?
Hi, I really thought you did a great job taking on this topic, you made it sound so simple which I was impressed by ! My question is just generally could you explain the Pcirf gene a little bit more and why you chose to study this specific gene ?
Thank you very much for a positive comment! That is a great question! So in fact we were the first group to study Pcirf gene and it did not have a name before we started our experiment. We have chosen this gene for two reasons – It belongs to Duf4246 domain which is a domain that represents genes that potentially express amino-acids that might have a role in DNA DSBs repair. The second reason why we used this particular gene is because it have very similar expression pattern to Rad51 which is known for having a role in DNA DSBs repair!
Did your gel controls consist of 1 negative control and 1 positive control group on the gel or were they both in the the same category?
Thank you for your question! So it in-fact contained one positive and one negative control group on the same gel. The positive controls were ftt18 to prove that the experiment was actually done properly and we that expected results would show in the well. The water was a negative control group to show if the samples were contaminated and we have expected that nothing would show up.
Can you explain a little more the importance of the Pcirf gene? Why was it the one picked?
Thank you for your question! Pcirf gene belongs to the DUF4246 domain that is a family of genes that potentially encodes proteins, this domain is not wildly reasserted by scientists and because of that it is important to discover what genes that belongs to it actually do because it might lead to a better understanding of a DNA DSBs which could directly help humans in treatment various diseases