8 thoughts on “C47 – Morrison

  1. Although your research did not yeild the results you were after could there be further research done as to why UPEC does not play a role in the repair process?

    1. There is! With the data we produced we can understand that UPEC does play a role in the DDR but it isn’t up-regulated during DNA damage. We can do a western blot to quantify the protein levels during the DDR to fully examine the levels during the repair process.

  2. Awesome job with your presentation. Do you think there are other model organisms you could used in future research that micmic Tetrahymena Thermophila or a model organism closely related to humans?

    1. Thank you! I believe that there are other model organisms that could be used in future research; although im not sure what they are specifically, I do know that T. Thermophila was used in this lab because it can be easily grown in this lab and its cheaper.

  3. Why was UPEC chosen over other genes expressed during DNA repair in tetrahymena thermophila reproduction?

    1. The gene UPEC wasn’t necessarily chosen, it was randomly given to my lab group and that was what we decided to name it. The gene hadn’t been studied by anybody before so it is a relatively new gene with no homologs or similarities to any other gene in a database.

    1. An RT-PCR is an experiment that is run the same way as a PCR to allow use to amplify and visualize the amplification of the UPEC gene. The difference is an RT-PCR is a reverse transcriptase PCR so the cDNA used is reverse trancripted into mRNA and then was looked at under treated vs. untreated conditions to view how the mRNA responds to the double stranded breaks in the DNA from the hydroxurea.

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