11 thoughts on “C5 – Carter

  1. What future experiments can be done to confirm the involvement of your gene in the DNA repair process?

  2. Could you elaborate more on how Tetrahymena Thermophila can be effective in repairing DNA damage since they induce DNA damage? Thank you 🙂

    1. Tetrahymena thermophila must be effective at repairing their DNA because they induce it in themselves. If they weren’t effective at repairing it, but induced it in themselves, they would certainly not be able to survive because they would constantly be suffering from the consequences of their DNA mutations.

  3. Odd question, but do you know why Tetahymena thermophila will induce DNA degrigation while reproducing?

  4. I was curious why the RPPO primer was used. I recognize it was used to test your primer’s uniqueness but how did the RPPO primer accomplish this?

    1. RPP0 was used in the first experiment as a positive control to confirm that if there were no bands in the lanes with designed primers, it wasn’t due to an experimental error. In the second experiment, RPP0 once again was a positive control to confirm no experimental error, and to confirm that expression didn’t change between the damaged and non-damaged cDNA for these 2 lanes because RPP0 doesn’t change expression during DNA damage.

  5. I probably already posted this question but I didn’t see it pop up on my screen but why does Tetrahymena thermophila degrade it’s DNA during reproduction?

  6. On the gel electrophoresis in the second figure, you point out that brightness is an indicator of DNA damage. However from visual inspection it looks as if the band in your experiment group is similar in brightness as your negative control. Does this mean that it had less potential damage than the positive control and was not overly expressed?

    1. Brightness is an indicator not of DNA damage, but of DNA expression of my gene. I was pointing out that the lanes with cells that DNA damage was induced had brighter bands indicating increased expression of the gene during DNA damage. And all three of the negative controls have no bands at all, as expected because there was no DNA added.

  7. What are some potential biological advantages to having a gene that changed expression during DNA damage? Can this only help the gene repair itself or could it make the damage worse overall?

    1. The implications of a gene increasing expression during DNA damage are that it could be involved in the DNA damage repair process. Once we know that the gene is involved in the DNA damage repair process, we can research whether or not it could be used in the diagnosis and treatment of disease. For example, it could be inhibited during chemotherapy because chemotherapy is purposefully inducing DNA damage in cancer cells, and the cells natural instinct to repair its DNA damage actually hinders this process.

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