Due to the results of this experiment, my hypothesis of the DNA damage response after conjugation would most likely be that the expression levels would be the same or not expressed at all in the damage induced Hypr2c gene.
In the Tetrahymena cells DNA damage is inevitable because the more cuts that are made the more errors are made when cutting the DNA, so there is no way to avoid it. DNA damage response is a network of pathways that are triggered when damage is sensed in a cell.
Hi Anna, I think that there could have been a mistake made in the PCR experiment. The results of the Ftt18 gene were inconclusive on many other students genes so there could have been an issue with the Ftt18 stock cDNA.
I don’t actually know the specific name of the repair factors but they are a crucial part of conjugation. The damage undergoes resection and then homologous DNA pairing where a strand of new DNA is inserted and repairs the damage.
I think that the inconclusive results could have been due to human error on my part when making the PCR or there could have been an issue with the DNA templates we used for Ftt18 and Hypr2c.
I can’t exactly say what went wrong with the second PCR it could have been the primers I designed or an error I made in synthesizing the cDNA. I think if I were to do this experiment again I would expect different results, but based on the other results for students with this same gene it could just not be expressed in this experiment.
Do you have any hypothesis as to what you would find in your research of the effects of conjugation on
DNA damage response?
Due to the results of this experiment, my hypothesis of the DNA damage response after conjugation would most likely be that the expression levels would be the same or not expressed at all in the damage induced Hypr2c gene.
Is there any possible way to limit or avoid DNA damage to the cell’s DNA when it is cut, and what is DNA damage response?
In the Tetrahymena cells DNA damage is inevitable because the more cuts that are made the more errors are made when cutting the DNA, so there is no way to avoid it. DNA damage response is a network of pathways that are triggered when damage is sensed in a cell.
Hi Ruby! Great presentation! What do you think could have caused the inconclusive results of your PCR that are being shown in your Ftt18 control?
Hi Anna, I think that there could have been a mistake made in the PCR experiment. The results of the Ftt18 gene were inconclusive on many other students genes so there could have been an issue with the Ftt18 stock cDNA.
What are some of those natural repair factors that Tetrahymena express?
I don’t actually know the specific name of the repair factors but they are a crucial part of conjugation. The damage undergoes resection and then homologous DNA pairing where a strand of new DNA is inserted and repairs the damage.
Hey Ruby. Did you have any limitations? Also what do you think caused the inconclusive results?
I think that the inconclusive results could have been due to human error on my part when making the PCR or there could have been an issue with the DNA templates we used for Ftt18 and Hypr2c.
What specifically went wrong with the PCR, and do you believe it would change the outcome if corrected?
I can’t exactly say what went wrong with the second PCR it could have been the primers I designed or an error I made in synthesizing the cDNA. I think if I were to do this experiment again I would expect different results, but based on the other results for students with this same gene it could just not be expressed in this experiment.