8 thoughts on “C51 – Rampy

    1. There were no bands likely because our gene (Pprs42) is not expressed during DNA damage. Another reason for this may be that we did not run enough cycles of PCR. Because our gene product is so small (108 bp) it may need more cycles of PCR to be visible. However, we can’t make a definitive answer if our gene is or is not expressed during the repair pathway.

    1. If Pprs42 were independent of the DNA damage repair pathway that would make future work a little harder. At least from what I know, it would be harder to narrow down where this gene would be involved if not in this pathway. It’s hard to pin down a pathway when just starting with a gene. One thing that could be done is using RNAi or CRISPR to knockdown the gene and see if this has any effects on growth and division. It could also be transiently expressed throughout the life cycle or just slightly increased during conjugation, we just don’t know right now.

  1. If your Pprs24 did work or if you studied another gene that did turn out to be involved in the pathway, how would that gene contribute to the knowledge about cancer or could is be used in some kind of treatment?

    1. As far as I know, this gene probably is not the best candidate for cancer research. As shown by our research, it is likely this gene is expressed in very low amounts to begin with. This suggests it is not too important in the repair pathway and would not be the best target for further research into cancer therapeutics.

    1. gDNA is genomic DNA while cDNA is complementary DNA. Complementary DNA is the DNA that represents the transcriptome. This is the product synthesized from mRNA during the reverse transcriptase PCR. gDNA is similar to cDNA in sequence, but it often contains introns that are spliced out during splicing. In the case for our gene, cDNA and gDNA were the same length. The size of the gene product is so small (108 bp) that there were no introns to be spliced.

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