4 thoughts on “C52 – Weingardt

  1. Just curious as to what you think might have caused the contamination shown in PCR gel electrophoresis in figure 4?

    1. Since the ATPV0C6 primers were contaminated with cDNA, the most likely cause was probably just experimental error, such as reusing pipette tips when distributing the cDNA and primers.

  2. In Figure 4, why did you use PCR gel electrophoresis for you experiments and why does lane 2 have a very dim glow and lane 3 and 4 have bright glows?

    1. Thank you for your question! We used PCR because it is a common method used to amplify DNA, specifically using primers. We would then be able to visualize the extent of this amplification under different conditions (in this case, using different primers) with gel electrophoresis. Lane 2 contains the cDNA amplified with the Cory gene-specific primers, so the dim band tells us that although the primer can anneal to the Cory gene within the T therm genome, the gene itself is poorly expressed. The bright band in lane 3 is the gDNA with gene-specific primers, which corroborates the idea that the cDNA (which directly correlates to the mRNA expression levels) isn’t as expressed as the gDNA. Lane 4 also has a bright band because in addition to the cDNA + ATPV0C6 primers, it also has more cDNA due to contamination, producing a brighter band at 302 bp.

Leave a Reply