Hey! Your presentation was really good and you were very knowledgeable about your research! My question for you is what were some unexpected challenges that you guys ran into during your research and how did you solve/overcome these challenges?
Good question! I was fortunate to have my research go fairly smoothly. The biggest challenge was in designing primers, I struggled to find a reverse primer that fit the criteria, so I ended up having to adjust and have a 19bp primer instead of 20bp. In the end, however, it did work!
Interesting question! My thought would be conducting similar experiments to this one, except with different functions. For example, trigger a different response (like stress or something, instead of DNA damage response), and then test to see whether mRNA levels increased.
Your presentation was great! Even though UbiconE2 was thought to have been DNA damage repair due to its characteristics, how would you find characteristics of certain genes that are used for DNA damage repair?
Good question. I think the most efficient way is to compare temporal expression profiles to that of genes known for DNA damage repair, as we did with UbiconE2, and then narrow from there.
Hard to tell! It’s named UbiconE2 based on its ubiquitin related activity, so likely something along those lines. This is pretty broad however, so I would assume its something related to protein regulation, as predicted by tools such as Ciliates.org
Hey! Your presentation was really good and you were very knowledgeable about your research! My question for you is what were some unexpected challenges that you guys ran into during your research and how did you solve/overcome these challenges?
Good question! I was fortunate to have my research go fairly smoothly. The biggest challenge was in designing primers, I struggled to find a reverse primer that fit the criteria, so I ended up having to adjust and have a 19bp primer instead of 20bp. In the end, however, it did work!
Nice presentation! What further experiments are going to be taken to narrow the results down?
Interesting question! My thought would be conducting similar experiments to this one, except with different functions. For example, trigger a different response (like stress or something, instead of DNA damage response), and then test to see whether mRNA levels increased.
Your presentation was great! Even though UbiconE2 was thought to have been DNA damage repair due to its characteristics, how would you find characteristics of certain genes that are used for DNA damage repair?
Good question. I think the most efficient way is to compare temporal expression profiles to that of genes known for DNA damage repair, as we did with UbiconE2, and then narrow from there.
Great presentation! What do you think the function of UbiconE2 is?
Hard to tell! It’s named UbiconE2 based on its ubiquitin related activity, so likely something along those lines. This is pretty broad however, so I would assume its something related to protein regulation, as predicted by tools such as Ciliates.org
What could be the rationale or explanation on a molecular level for why your results came back completely inverse to what was expected?
Great Question. I think the answer to that is that simply put, it is not involved in this process of DNA damage repair.