15 thoughts on “C54 – Anderson

  1. If UPEC is not involved in the damage response then what are ways we can help repair damaged cells to prevent things like cancers?

    1. Hello! Gel electrophoresis is a way of visualizing the PCR samples we created. The way this works is the DNA is overall negatively charged so when exposing it to an electric current, it will run down the gel toward the positive end. The distance traveled is proportionate to the size of the DNA.
      Thanks!
      Eva

    1. Hi! We don’t necessarily hypothesize that it will, however, we believe it is important to replicate the experiment under more than one condition to be able to say for certain if Upec is or is not involved.
      Thanks,
      Eva

  2. What leads you to believed that UPEC will be present in damage done through other methods? (sorry if I commented twice my comment wasn’t showing up so I wasn’t sure if I commented.)

  3. How exactly did you induce damage to analyze the difference between expression levels? Great project and very interesting1

    1. The damage was induced by Hydroxyurea (Hu) which we chose for its well known cytotoxicity.
      Thanks!
      Eva

    1. Hello! If Upec is not in DNA damage response, that is still valuable information. At this point in time, we do not know much about Upec and it has no known homologues (similar chromosomal structure). The knowledge of whether it is involved in this function adds to what we know about this gene and also can allow us to focus on other genes of interest in future DDR experiments.
      Thanks,
      Eva

  4. If you are manually inducing DNA damage, why does it matter if the model organism has high natural DNA damage?

    1. Hi! The model organism is used in place of the human genome. Since we ultimately care about these genes in humans, we had to find an organism that would respond similarly to how our own genomes would.
      Thanks,
      Eva

  5. super interesting and great presentation! what other methods of damage can be used for future directions?

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