8 thoughts on “C55 – Bachu

  1. Great job with your presentation. One question I had was that in figure one for your PCR reading you said that the primers you used didn’t show up when the primers were analyzed. Why do you think this happend? was it experimental error or the overall primer was just not effective?

    1. Thank you so much! To answer your question, we believe that the PCRs were set up incorrectly, we just didn’t add any primers to our PCR tubes, or the primers degraded. If we had added the primers, then we would have at least seen primer dimers at the bottom of the gel, but since we didn’t, we concluded that either the primers weren’t added or that they had degraded thereby not amplifying our gene of interest.

  2. I though this was an amazing presentation and many of the details were explained thoroughly. My only question is what would be the real world application of this? I understand what Rrm 2 is but how can we use it?

    1. Thank you! As far as real world applications go, understanding Rrm2 is just a stepping stone to better understand the DNA damage response pathway. If we can learn more about the proteins that play a role in this pathway, then it is much more likely that we can use those proteins to better the DNA damage response in other organisms such as humans. This way, if our DNA does mutate and break, then we can correct before any larger issues come to light, such as the onset of cancer.

  3. Amazing presentation and I think that you covered the basis very well in an engaging and though way. My only question is, how is this being used to develop medicine?/ What do these findings mean in the big picture?

    1. Thanks so much! This research could be used to develop medicine because of the impact it has in better understanding the DNA damage response pathway. Knowing exactly what role Rrm2 plays leads us closer to finding ways to improve the DNA damage response mechanism is humans. Hopefully, understanding this protein will lead to determine why T.Therm is so accurate in repairing DNA damage. With that information, we can use similar processes to effectively and efficiently improve DNA damage in humans

  4. This was such a great presentation. I wanted to know, how were you able to extract the RNA in your experiment? This presentation was very well prepared, and you spoke very clearly. Great job!

    1. Appreciate it! In order to extract RNA, we basically lysed the cells, then ran all the contents of the lysed cell through a silica column. In this column, the negatively charged RNA stuck to the silica while all the other parts of the cell were rinsed out. Then we released the RNA from the column to complete isolating it. We did this for both the undamaged cells and cells that were damaged with hydroxyurea.

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