12 thoughts on “C59 – Desousa

  1. Great job on your presentation! This is a complicated topic but you articulated it well. My question is: I don’t believe you said anything about a negative control (sorry if I did happen to miss it) so I was curious why your study didn’t need one

  2. Very good presentation! I do not know a ton about this specific field so I am curious if there are other genes that you could do this same study on as well?

    1. Thank you! There are plenty of other genes in the Tetrahymena thermophila genome that have not yet been characterized in terms of function, so these experiments can be performed in future semesters on different genes of interest in the genome.

    1. Thank you! As Cory has a ribonuclease domain, we have hypothesized that it functions to degrade RNA during conjugation, possibly mRNA transcripts after translation as a lot of different genes in T. thermophila are highly expressed during the process.

  3. Well done! Although your results all seem to indicate that Cory has close to no function regarding DNA repair, what do you theorize could be some other reasons as to the high expression in conjugation?

    1. Thank you! As one of Cory’s conserved domains is a ribonuclease domain, we hypothesized that it may function during conjugation to degrade RNA, specifically mRNA transcripts after they have been translated, as a large number of genes are highly expressed during conjugation, thus a large amount of mRNA transcripts are produced.

  4. Hey Kayla, great job! Do you have any ideas about what other roles Cory might play in these events? It would be interesting to find out why it has a higher expression during conjugation!

  5. Hi Kayla, could you explain a bit more what conjugation is and why it is relevant to DNA repair?

    1. During conjugation, the two participating Tetrahymena thermophila cells undergo almost complete deconstruction and reconstruction of their genomes. This extreme amount of DNA damage has to be regulated by a very robust DNA damage repair pathway in order to prevent the cells from dying during the reproduction process.

    1. We used a bioinformatics tool called Primer3Plus to help us design primers that had a high probability of annealing specifically to our gene to replicate it during PCR.

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