6 thoughts on “C6 – Engelke

  1. Great presentation! Why do you think there was not a large increase with the addition of HU? Was this what you were expecting?

    1. One of the reasons there wasn’t a large increase in transcription after HU induced damage me be that Cory is expressed at low levels. This may also be why there is no band in the cDNA for Cory in the primer validation gel.

  2. What exactly might you do once you figure out the protein function with the T.thermophila genome

    1. While medical uses like a preventative or reparative cancer treatment are potential uses for a DNA damage repair protein, it would be interesting to see if we could use a protein involved in conjugation to help induce mutations. This thought comes to mind because during conjugation two T.therm effectively interchange DNA.

      In a perfect world you could offer a plasmid with a gene specific mutation that is otherwise identical to your target plasmid and make conjugation proteins do the heavy lifting.

  3. Great job! what would the process for confirming and establishing the protein’s function look like and how would you go about noting additional characterizations?

    1. The process for establishing the protein’s function would likely start by trying to induce a mutation within Cory for a florescent protein. Then by following a similar process to primer conformation to confirm mutation with the ultimate goal of seeing where the protein localizes after DNA damage. This my give insight as to whether the protein is responsible for signaling or directly responsible for DNA damage repair.

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