Great job! After determining the definitive function of UbiconE2 would be interested in conducting more research surrounding UbiconE2? What other experiments could you possibly conduct after determining the function of UbiconE2?
It would probably depend on what that function turned out to be if further research would be beneficial. The same would apply to your second question. Depending on what the function is, more experiments could be conducted determining the role that function plays in other cellular processes. Again using something like gene knock out or protein localization during different cellular processes. Another experiment could be cluster analysis that, which would essentially be using the known function of UbiconE2 to determine functions of other genes.
Do you have a prediction about why the control wasn’t working within the entire class? Also, could the gene have any sort of role within the organism when DNA is damaged because it still has some expression?
I am not entirely sure what went wrong with the Ftt18 control. My guess would be varying levels of Ftt18 cDNA/mRNA that could have resulted from experimental error/mRNA degradation.
Gene knockout is deliberately inhibiting the function of a gene and observing the effect that has on the cell. It determining how the absence of a gene affects cellular function, the function of the gene itself is revealed. Gene knockout can be achieved through something like CRISPR-cas or inducing mutations.
I apologize, this response somehow shifted to the question below you:
Gene knockout is deliberately inhibiting the function of a gene and observing the effect that has on the cell. It determining how the absence of a gene affects cellular function, the function of the gene itself is revealed. Gene knockout can be achieved through something like CRISPR-cas or inducing mutations.
Isolating parts of UbiconE2 would imply determining its protein structure and its functional domain(s). I concluded UbiconE2 most likely does not play a role in DNA repair, however, determining its protein structure would be beneficial in understanding it’s definitive role in the cell and other cellular processes.
Why do you believe that there was an overall decrease in gene expression and how do you think this would have changed your experiment if you saw in increase in gene expression?
It is very difficult to see on the poster, but the band for UbiconE2 in the DNA damaged lane is actually dimmer than the band in the untreated DNA lane. A dimmer band means less mRNA was produced, thus indicating a decrease in expression. The decrease was also confirmed via quantitative analysis which showed the expression of UbiconE2 was actually ~10% lower under DNA damage. An increase in expression would have increased my confidence that UbiconE2 could potentially play a role in DNA repair, and further exploration down that avenue would be garnered.
Great job! After determining the definitive function of UbiconE2 would be interested in conducting more research surrounding UbiconE2? What other experiments could you possibly conduct after determining the function of UbiconE2?
It would probably depend on what that function turned out to be if further research would be beneficial. The same would apply to your second question. Depending on what the function is, more experiments could be conducted determining the role that function plays in other cellular processes. Again using something like gene knock out or protein localization during different cellular processes. Another experiment could be cluster analysis that, which would essentially be using the known function of UbiconE2 to determine functions of other genes.
What is gene knockout and how would that assist in figuring out the function of UbiconE2?
Do you have a prediction about why the control wasn’t working within the entire class? Also, could the gene have any sort of role within the organism when DNA is damaged because it still has some expression?
I am not entirely sure what went wrong with the Ftt18 control. My guess would be varying levels of Ftt18 cDNA/mRNA that could have resulted from experimental error/mRNA degradation.
Gene knockout is deliberately inhibiting the function of a gene and observing the effect that has on the cell. It determining how the absence of a gene affects cellular function, the function of the gene itself is revealed. Gene knockout can be achieved through something like CRISPR-cas or inducing mutations.
I apologize, this response somehow shifted to the question below you:
Gene knockout is deliberately inhibiting the function of a gene and observing the effect that has on the cell. It determining how the absence of a gene affects cellular function, the function of the gene itself is revealed. Gene knockout can be achieved through something like CRISPR-cas or inducing mutations.
Why does your model organism intentionally induce DNA damage to its own genome just to repair it?
I believe it does so to exchange genetic material with its partner, to produce genetic variability in offspring.
Is there a way to isolate the parts of UbiconE2 that only repair DNA damage?
Isolating parts of UbiconE2 would imply determining its protein structure and its functional domain(s). I concluded UbiconE2 most likely does not play a role in DNA repair, however, determining its protein structure would be beneficial in understanding it’s definitive role in the cell and other cellular processes.
Why do you believe that there was an overall decrease in gene expression and how do you think this would have changed your experiment if you saw in increase in gene expression?
It is very difficult to see on the poster, but the band for UbiconE2 in the DNA damaged lane is actually dimmer than the band in the untreated DNA lane. A dimmer band means less mRNA was produced, thus indicating a decrease in expression. The decrease was also confirmed via quantitative analysis which showed the expression of UbiconE2 was actually ~10% lower under DNA damage. An increase in expression would have increased my confidence that UbiconE2 could potentially play a role in DNA repair, and further exploration down that avenue would be garnered.