Why was FA2H chosen as the subject of testing for DNA damage repair specifically. Are there any main key factors that make it a better fatty acid to test for DNA damage repair in comparison with something else?
The primers were chosen by looking at the DNA in the gene and selecting primers based on how well they could anneal to the DNA, as well as GC content and the temperature that the primers could withstand. A different gene would require different primers, so it is very unlikely that my primers would anneal to any other gene.
My best guess for this result is that there was a pipetting error, meaning unequal amounts of Ftt18 template DNA made it into the gel, though this result may have occured for a number of reasons.
The FA2H gene product is responsible for adding a hydroxyl group to fatty acids, and is also involved in synthesizing the myelin sheath in nerve cells.
The purpose of this was really just to see if I could design my own primers to anneal to the gene. The validated primers were a backup in case my primers didn’t work, which is somewhat common and ended up being the case for me.
The FA2H gene product is responsible for adding a hydroxyl group to fatty acids, and is also involved in synthesizing the myelin sheath in nerve cells.
Why was FA2H chosen as the subject of testing for DNA damage repair specifically. Are there any main key factors that make it a better fatty acid to test for DNA damage repair in comparison with something else?
How did you choose the primers you did? Do you think the primers you chose would have annealed to the DNA if a different gene were being examined?
The primers were chosen by looking at the DNA in the gene and selecting primers based on how well they could anneal to the DNA, as well as GC content and the temperature that the primers could withstand. A different gene would require different primers, so it is very unlikely that my primers would anneal to any other gene.
Great video! Do you know what might’ve caused the decrease of gene expression of the FTD-18 gene?
My best guess for this result is that there was a pipetting error, meaning unequal amounts of Ftt18 template DNA made it into the gel, though this result may have occured for a number of reasons.
What function do you think this gene product has if not DNA repair?
The FA2H gene product is responsible for adding a hydroxyl group to fatty acids, and is also involved in synthesizing the myelin sheath in nerve cells.
What other experiments would you like to perform that would allow you to see the link between DNA damage and neurodegenerative diseases?
What was the purpose of using primers of your design if there are primers known to anneal to the FA2H gene?
The purpose of this was really just to see if I could design my own primers to anneal to the gene. The validated primers were a backup in case my primers didn’t work, which is somewhat common and ended up being the case for me.
The FA2H gene product is responsible for adding a hydroxyl group to fatty acids, and is also involved in synthesizing the myelin sheath in nerve cells.