View the poster here. Share this:Click to share on Twitter (Opens in new window)Click to share on LinkedIn (Opens in new window)Like this:Like Loading...
12 thoughts on “C62 – Kumar”
Which process would you favor further in, with knockout or immure precipitation?
Nuftr-1 has a domain called NHP6B family domain which is involved in RNA Pol III function so I would start with that.
After you are done with the further directions, would you be willing to continue working with Nuftr-1 or would you be interested in looking into other genes?
If I am focusing on DNA Repair, I would stop looking into Nuftr-1.
Why did you choose knock out and immunoprecipitation
They are the best ways to determine gene functionality.
How would immunoprecipitation help you identify what proteins Nuftr-1 interacts with?
It will allow us to identify other proteins that interact/bind to Nuftr-1. If we know the processes that those proteins are a part of, we might identify Nuftr-1’s function.
It will identify proteins that Nuftr-1 interacts with. If we know the function/processes of the proteins identified, we might know Nuftr-1’s function or at least the processes it is a part of.
What was your your favorite part of the experiment?
Do we have access to crisper/cas9 tech at CU Boulder?