6 thoughts on “C62 – Hirano

    1. We chose to study DNA repair because of the importance of this process in eukaryotic life. DNA is reactive and can be easily damaged, which results in cell death, mutations, and disease. A better understanding of DNA repair mechanisms can lead to potential advances for human health.

  1. hi, can you explain this a little bit further? like why “Upec not likely to be involved in DNA damage response”

    1. To be involved in the DNA damage response we would expect the expression of Upec to increase following the addition of hydroxyurea (which induces DNA damage). An increase of expression is represented by a brighter band in the RT-PCR (Figure 3). Since the RT-PCR Upec band following the addition of hydroxyurea is darker than the band without DNA damage, this would suggest Upec’s expression does not increase in response to DNA damage and is likely not involved in the DNA damage response.

  2. Hi, did you have a choice when choosing your compound? If so, why did your group choose Upec to see if it was involved in DNA damage?

    1. No, we did not have a choice – the genes were randomly assigned.

      However, Upec had the potential to be involved in the DNA damage repair process. From Figure 1 on the poster, Upec has a similar expression profile to a gene called Rad51. Rad51 is well known for its involvement in DNA damage repair and has a distinct two-peak gene expression profile. Many other genes involved in DNA damage repair also have this expression profile. This allowed us to hypothesize that Upec might be involved in the DNA damage repair process.

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