9 thoughts on “C66 – Yu

  1. Hi JiaWei great poster! You said in there was no obvious increase in gene expression with the treatment; I’m curious were you originally expecting it to be increased, or did you find the results to aline with with your previous knowledge and/or a previous hypothesis?

    1. We were initially expecting the gene expression of Setk to be increased after DNA damage because Setk expression was increased during conjugation (Figure 2), a phase in the Tetrahymena life cycle characterized by DNA damage and repair.

  2. Hey there the presentation was great, I did have one question though. What was the reason for the spread on the bands in the RppO section of Figure 3?

    1. For this question, I am assuming you mean the spread between the band in the third land and the band in the fourth lane. The third lane corresponds to Rpp0 with a cDNA template (Without introns) and the fourth lane is with Rpp0 and a gDNA template (With introns). The presence of introns will cause the resulting PCR product to be longer than without introns, hence the spread between the bands. There is no spread between lanes 1 and 2 because the Setk gene had no introns in it, so the cDNA and gDNA lengths were the same size.

    1. A gene being upregulated means that it has increased gene expression. Setk being upregulated during conjugation means that Setk has increased gene expression during the conjugation phase of the T. thermophila life cycle.

  3. Hello!

    I was wondering what does conjugation mean and why it is important in regards to your hypothesis?

    1. Conjugation is a specific phase of the T. thermophila life cycle notable by the two peaks found in figure 2. Specifically, it is a reproductive stage of its life cycle that includes meiosis and self induced DNA damage. The fact that self induced DNA damage and repair is in this stage is what spurred us to research Setk’s role in DNA damage repair.

  4. Hello!

    Why was Tetrahymena thermophila chosen as the gene of interest? How does it cut up it’s own genome?

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