In the process of DNA damage other proteins may be needed to handle the damaged environment. This will take more energy for the cell to translate and transcribe the genes needed to compensate for DNA damage, therefore unnecessary genes will be down-regulated to direct energy where it is needed.
I would assume it was from some sort of human error. RNA extraction and cDNA synthesis are tricky experiments as RNases are everywhere! So it’s a little tricky to make sure the RNA isn’t degraded during the experiment. If the RNA was degraded reverse transcription couldn’t occur and no bands would be present.
Since there was little to no change in Nuftr1 gene expression in the presence of DNA damage, inducing it to other environments might show what conditions it could be up-regulated in giving us insight to its function in the cell.
Yes! We used a couple programs including Primer3Plus, Benchling, and Cilitates. First, I found the gene sequence of Nuftr1 on Ciliates. Then through Primer3Plus and a specific set of criteria we designed the primers. The criteria included length of primers, melting temperature, and GC content. The primer designs were sent to a company to synthesize the primers.
According to other people results, NTF 1 had a downregulation or no change in gene expression. Do you know why that is?
In the process of DNA damage other proteins may be needed to handle the damaged environment. This will take more energy for the cell to translate and transcribe the genes needed to compensate for DNA damage, therefore unnecessary genes will be down-regulated to direct energy where it is needed.
Why was there no bands in the Nuftr1 section of the gel electrophoresis?
I would assume it was from some sort of human error. RNA extraction and cDNA synthesis are tricky experiments as RNases are everywhere! So it’s a little tricky to make sure the RNA isn’t degraded during the experiment. If the RNA was degraded reverse transcription couldn’t occur and no bands would be present.
what is the benefit of exposing your model organism to other environments
Since there was little to no change in Nuftr1 gene expression in the presence of DNA damage, inducing it to other environments might show what conditions it could be up-regulated in giving us insight to its function in the cell.
Did you actually make those primers by analyzing the gene sequence? If so, I would to love more about the process!
Yes! We used a couple programs including Primer3Plus, Benchling, and Cilitates. First, I found the gene sequence of Nuftr1 on Ciliates. Then through Primer3Plus and a specific set of criteria we designed the primers. The criteria included length of primers, melting temperature, and GC content. The primer designs were sent to a company to synthesize the primers.