Super interesting topic! Do you know what mechanism Tetrahymena uses to cause the breaks in the DNA, and where it might cause those breaks? Is it possible that UbiconE2 is involved in DNA repair in Tetrahymena, and that Tetrahymena causes breaks in it’s DNA in patterns that are recognizable to UbiconE2?
Hi Erin! I am not sure of the exact mechanism, but it uses the gene Spo11 to induce the breaks. It is possible that UbiconE2 still may play a role in DNA repair, but based off of my data I think that it likely plays a different role.
Good job. I’m sorry your genes don’t work out. I found that your genes were not expressed at all. Maybe there is something wrong with primer. Is it possible? Keep going
Hi Chengwei! It was unfortunate the experiments did not go as planned, but there is still much more to investigate! There is a possibility that the lack of bands was due to an issue with either the primers or the PCR set up as well.
Super interesting topic! Do you know what mechanism Tetrahymena uses to cause the breaks in the DNA, and where it might cause those breaks? Is it possible that UbiconE2 is involved in DNA repair in Tetrahymena, and that Tetrahymena causes breaks in it’s DNA in patterns that are recognizable to UbiconE2?
Hi Erin! I am not sure of the exact mechanism, but it uses the gene Spo11 to induce the breaks. It is possible that UbiconE2 still may play a role in DNA repair, but based off of my data I think that it likely plays a different role.
Hi, I was wondering if you knew the reason behind why Tetrahymena induce double stranded DNA breaks when undergoing conjugation?
Hi Ryan! Tetrahymena induces the breaks in order to engage in genetic cross over with the other organism and increase the genetic diversity.
Hi this was really interesting. I was just wondering what you might think UbiconE2 is most likely involved in if not DNA repair?
Hi Pauline! I think that UbiconE2 likely plays a role in ubiquitin mediated degradation due to the fact that it is a ubiquitin conjugating enzyme.
I really liked your presentation! What do you think UbioconE2 functions as?
Hi Abby! Thank you, I think UbiconE2 likely plays a role in ubiquitin mediated degradation.
Good job. I’m sorry your genes don’t work out. I found that your genes were not expressed at all. Maybe there is something wrong with primer. Is it possible? Keep going
Hi Chengwei! It was unfortunate the experiments did not go as planned, but there is still much more to investigate! There is a possibility that the lack of bands was due to an issue with either the primers or the PCR set up as well.