6 thoughts on “C70 – Kasayapanand

  1. Great work on your presentation Minerva! Was there any larger context the gene is relevant in? Like in medicine. Could it be of therapeutic relevance?

    1. I guess I meant in terms of previous work done. Like what motivated study of it in the class?

      1. fondly enough we got to name the gene and there wasn’t all that much known about it, when we needed to name it we needed get some info about it using BLAST and cilia.org to figure out what it was and the most it could tell us was that is coded for a lot of leucines and a portion of it had a phosphate regulation domain hence the name “protein phosphate regulatory subunit 42” – pprs42, the description for the domain talked a little bit about it allowing for the seperation of chromosomes but we can’t say whether that is what the gene in the context of this experiment does. We just know from these experiments is that Pprs42 is not expressed under normal conditions (no cDNA band during primer validation) and in the context of DNA damage it’s still not expressed (no bands in UT or HU lanes) I hope this answers your question 😀

  2. Hi Minerva! Good job on your presentation and poster! One thing I’m curious about is what is meant by “the C7 and C17 phase of conjugation” and how did the peaks lead to the assumption that Pprs42 may play a role in DNA damage?

    1. C7 and C17 are phases of conjugation, conjugation is the process of which T.therm tears apart it’s DNA and recombines it during reproduction we thought that the peaks showing clear high gene expression at these points of reproduction would mean that the gene would would play a role in DNA repair

  3. sadly the gene doesn’t have any human homologs, the gene we found most similar to the T.therm gene was found in a worm. As far as therapeutics is concerned the purpose of the experiment is to better understand and find genes related to the DNA repair mechanism. Even if there isn’t a gene that’s identical or very similar to this gene it’s possible there’s portions/traits of Pprs42 that might be similar such as a noted leucine rich repeat feature that could play some other role related to cell growth or signalling. despite Pprs42 not being expressed in response to DNA damage

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