8 thoughts on “C71 – Koehler

  1. The results in figure 2 are shown to be inconclusive, however, what would the possible expected results be?

    1. If there was increased gene expression, you would expect a brighter band at the same bp in the HU lane. If there was no change in expression, you would expect bands of approximately equal intensity in both UT and HU lanes. Additionally, dimmer or no bands in the HU lanes could indicate decreased expression under DDR conditions.

  2. Is the target gene present in the human genome? If so, how big of a role does it play on cancer prevention?

    1. There are no known homologs in the human genome, however there is indication that Hycop plays a role in T Therm pathways, meaning it could potentially be utilized in cancer prevention.

  3. It was stated that there was a problem with the untreated DNA causing inconclusive results. What sort of error could have caused this problem?

    1. RNA is a highly unstable molecule and is easily degraded outside of cellular conditions. RNAses exist naturally on human skin, thus contamination could have this result.

  4. Do you think results would be different had you used a different reagent to induce double stranded breaks? Why did you choose Hydroxyurea?

    1. Results could potentially differ given that other reagents include DBS differently and with different intensity of effect. However, if Hycop were to show increased expression under hydroxyurea damage conditions, it would likely show similar expression using different DBs reagents.

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