6 thoughts on “C72 – Ahmed

    1. We designed our primers using benchling software to visualize the whole tetrahymena genome. In the genome, we later identified the gene we would be studying which is rrm2. Then using the coding sequence of our gene, we designed our primer that can properly anneal to the target DNA.

  1. Said that the forward and reverse primers were successful because it binded to the cDNA and gDNA at different positions and sizes. What specifically is the bp size/length that confirms this?

    1. The size/length of both forward and backward primers was 286 bp. This was obtained by going through the cDNA and gDNA of our RRM2 gene. A perfect region was found in the gene that has complementary base pairs that align with our primers.

  2. Great presentation! My question is how would you go about “fixing” the expression of RM2 in cells that lack it.

    1. Great question! We have to access whether our gene expression will be highly expressed in response to DNA damage or not when integrated into other organisms. We can also create the transcription factors in the organisms that lack this gene to enhance the expression of this gene when integrated.

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