8 thoughts on “C72 – Lucas

  1. If not DNA damage repair, do you have any theories as to how this gene impacts the conjugation process?

    1. Since this gene hasn’t been widely studied before, it is difficult to obtain information on how it impacts conjugation. However, to further look into this function, Gene Knockout experiments using CreLox could be used to make our gene, Pprs42 unfunctional and then observe the effects it has on an organism. It may be detrimental to the organism to knock this gene out if it is involved in a critical part of conjugation as the organism may no longer be able to reproduce.

    1. The DNA we used was from our model organism Tetrahymena thermophila and using PCR we could see if our gene Pprs42 was involved in the DNA damage repair response by using it as the template DNA.

    1. The contamination led to multiple bands being displayed in the PCR lanes, which without having the predicted sizes of our gene specific gDNA and Rpp0 gDNA could have been confusing for us to interpret. Luckily, Benchling gave us the predicted sizes of our bands and we did have bands at the lengths listed so we could confirm our primers did in fact anneal to the target DNA. It seemed as if cDNA could have been added to all tubes in error, except the water lane for the gene specific lane. Therefore, this contamination did not have major effects as we could still see our gene specific primers annealed at the correct lengths, however other contamination could have impacted our results and therefore we would have had to use the validated primers to continue our research project.

  2. Based off of this, what would you hypothesize is the function of this gene if not DNA repair?

    1. We have found that this gene is involved in conjugation which is a reproductive process in organisms. We could investigate further functions of this gene by using gene knockout experiments and also protein localization experiments to show us where this gene acts in the organism which could help us to determine further functions.

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