8 thoughts on “C74 – Matthews

  1. Are there any other characteristics behind tetrahymena thermophila the ideal model organism besides that it can undergo reverse transcriptase?

    1. Hi Chase! I’m not sure I understand your question… The role of reverse transcriptase is to convert RNA into cDNA. This is not what makes Tetrahymena thermophila a good model organism. What makes it a good model organism is that it cuts up its genome every time that it mates so its constantly uses DNA damage repair pathways. We also have a lot of bioinformatic data on their genes, which has allowed us to study so many. It’s also easy to grow.

  2. Are the DNA damage pathways specific to tetrahymena thermophila, if so, what makes it such a good candidate for study?

    1. Hi Ella! DNA damage pathways are not specific to Tetrahymena thermophila, every living organism must use these pathways to survive! But there are still many other things that make it a good candidate to study. This organism cuts up its own genome every time that it mates so therefore often utilizes these repair mechanisms. We also have a lot of bioinformatic data on their genes which has allowed our class to study so many different genes in Tetrahymena. Lastly they are easy to grow!

    1. Hi Manas! Tetrahymena thermophila was an appropriate model organism for this lab because they actually cut up their genome every time that they mate, so they are constantly repairing DNA damage. We also have access to a ton of bioinformatic data which has allowed us to study so many of their genes. They are also easy to grow!

    1. Hi Alex! To induce DNA damage into the model organism, Tetrahymena thermophila, we used the DNA damaging reagent hydroxyurea. The goal was to see if Hycop had a role in repairing the damage that was induced. We could not draw conclusions about whether or not Hycop was involved.

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