7 thoughts on “C77 – Bickner

  1. Hi Mayu! Im curious how BLAST! is able to tell you if your primer is viable or not. What does it look for?

    1. Hello!! BLAST is short for Basic Local Alignment Search Tool, and essentially finds the regions of similarity between sequences. It compares protein or nucleotide sequences in its database and calculates the statistical significance of matches. Essentially, we ran the sequence for the primers that we have designed for our gene in this program, and then the program compared that sequence to all the sequences in the data base, and showed whether or not the primer sequence will have a high chance of annealing to other genes based on e-values(the lower the e value, the less likely it will anneal to other genes). Our primer only showed affiliation to our specific gene, and had very low e values for all other genes, demonstrating that it was a good fit for our specific gene.

  2. Hey!! How would you/could you use a different/more specific primer in future experiments to verify your results?

    1. Yes it is possible to use different primers in the future, however, our experiments demonstrated that our primer is specific to our gene, and functions well, so I do not see a reason to redesign another primer, especially for the objective of the experiments.

  3. Hey!! How could you/would you use a different or more target-specific primer to verify your results in future experiments?

  4. Would it be beneficial to perform tests to find out the roles of Rfc1 outside of its involvement in the DNA repair pathway?

    1. Yes!! I believe it will be very beneficial to understand the other functions of Rfc1 outside of its involvement in the repair pathway. Specifically, my group discussed that due to the minimal involvement of the gene in the repair pathway, and the gene similarity to the human gene for replication factor C subunit 1. I believe that the gene knockout method will demonstrate the bigger functions of the gene in the cell. Understanding different effects of genes is always important in better understanding the internal cell functions

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