12 thoughts on “C8 – Miner

  1. Hi Meaghan! Great presentation! I was wondering in figure one why did you use the primers that you used in PCR for your experiment?

    1. Hello Bridget, thats a great question! I did a lot of research with understanding the gene using Benching and other sources, and was able to go through homologs and domains to find two primers that would be possibly sufficient for my gene. I went with the primer I did because it framed an intron.

  2. Is Tetrahymena thermophilia a type of gene or is it an organism that you use the thermophila gene for DNA damage reapair? I really enjoyed your presentation!

    1. Hello Anna! Tetrahymena is a microscopic organism! Here is a link that goes more in depth (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132220/), but a quick over view is that it can be found all over the world in basically just about any environment and are super easy to maintain in a lab setting. They are well researched so they are a common model organism for molecular research.

    1. Hi Heramb! I belive there could have been a problem with the wells in my gel in Figure 4, Gel 1, Lane 2 and Lane 1 that could have had the ladder bleed over based off i had a similar problem in Lane 8-10, which is why there is a second Gel.

  3. Awesome presentation! Do you know what exactly was involved in the primer design for your research?

  4. Hi Meaghan, great work! You mentioned that if you were to repeat the experiment, you would use a different gene. What other gene(s) are possible DNA repair factors?

    1. Hi Brandt great question! I would repeat the experiment with genes that show a similar two-peak expression at conjugation because that is usually a great indicator that it is being expressed in response to damage to the DNA.

  5. How does Tetrahymena thermophila undergo DNA damage when it replicates? Awesome presentation btw!

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