8 thoughts on “C81 – Hattendorf

    1. We chose ATPV0C6 as our control because it’s whats known as a house keeping gene. This means it’s expressed at the same level under all conditions which means every band should ideally be the same intensity under any condition we test it in which makes it an ideal loading control to compare things too!

  1. Great presentation with clear results, how were you able to decide the control to compare it to the upec?

    1. We chose ATPV0C6 as our control to compare to Upec because its known as a housekeeping gene. Which is a gene that is expressed at the same level under all conditions, which would mean, as a loading control, it would ideally have the same brightness for each band. This makes it ideal as a control and as comparison for quantitative analysis.

    1. Figure.1 our primer validation gel allows us to validate our primers because we can predict the length of the PCR product on our gel. Our primers are validated when we see bands at our expected lengths, indicating that our primers properly anneal and amplify our gene of interest.

  2. How do you think the future directions and research will show Upec’s certain function in T. thermophilic?

    1. Future experiments can allow us to look into Upec’s function, experiments that allow us see protein structure can give us some incite into possible function. Gene knockout experiments can also allow us to see what happens to T. Therm when Upec is nonfunctional which can also be indicative of function.

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