This is such an interesting topic! If you were to continue and find out the function of Wcop40 what would that information lead you to in terms of future experiments or research?
We used the different DNA conditions to evaluate how DNA damage alters gene expression. Hydroxyurea is a damaging agent, so the +HU samples were compared to the untreated samples to observe how gene expression changed after DNA damage.
This is such an interesting topic! If you were to continue and find out the function of Wcop40 what would that information lead you to in terms of future experiments or research?
Once we know the function of the gene, we could knock it out using CRISPR to see what other cellular processes it is involved in.
Why did you use two DNA conditions for Rad51 and ATPvoc6? What is the difference between using untreated DNA and DNA treated with Hydroxyurea?
We used the different DNA conditions to evaluate how DNA damage alters gene expression. Hydroxyurea is a damaging agent, so the +HU samples were compared to the untreated samples to observe how gene expression changed after DNA damage.
How and why did you chose your negative controls?